Fig. 1.
Fig. 1. Multiple hematopoietic lineages generated from in vitro-differentiated ES cells on OP9 stromal cells (ES-cell/OP9 coculture). / Hematopoietic lineages were characterized from 14-day ES-cell/OP9 cocultures by cell surface expression of lineage-specific markers. (A) Staining with control monoclonal antibodies (mAb) is shown in the left panel. In the right panel, 4 distinct populations, labeled a-d, were detected by flow cytometry on the basis of CD45 and CD24 surface expression. (B) Populations a-d were further characterized by lineage-specific markers for erythrocytes (a, TER119+); myeloid cells (c, CD11b+); B lymphocytes (b, CD45R+); and natural killer (NK) lymphocytes (d, DX5+CD90+).

Multiple hematopoietic lineages generated from in vitro-differentiated ES cells on OP9 stromal cells (ES-cell/OP9 coculture).

Hematopoietic lineages were characterized from 14-day ES-cell/OP9 cocultures by cell surface expression of lineage-specific markers. (A) Staining with control monoclonal antibodies (mAb) is shown in the left panel. In the right panel, 4 distinct populations, labeled a-d, were detected by flow cytometry on the basis of CD45 and CD24 surface expression. (B) Populations a-d were further characterized by lineage-specific markers for erythrocytes (a, TER119+); myeloid cells (c, CD11b+); B lymphocytes (b, CD45R+); and natural killer (NK) lymphocytes (d, DX5+CD90+).

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