Colocalization of AF10 and GAS 41 endogenous proteins in KG1a cells by immunofluorescence.

(A) Subcellular localization of AF10, detected with a FITC-conjugated secondary antibody. (B) Subcellular localization of GAS41, detected with a Cy3-labeled secondary antibody. (C). Overlay of the previous images showing colocalization of the 2 proteins, both in the cytoplasm and in the nucleus. (D) Phase contrast image of the same field, showing the presence of cell debris.