Fig. 4.
Effect of ristocetin or botrocetin concentration on vWF binding to GPIbα.
Specific binding of 125I-labeled vWF (1 μg/mL) to CHO βIX cells (106/mL) expressing wild-type human GPIbα or the C249-268 chimera in the presence of ristocetin (A) or botrocetin (B) at the concentrations indicated for 30 minutes at 22°C. Specific binding was calculated from total binding by subtracting nonspecific binding measured in the absence of modulator in parallel assays.
