Binding of monoclonal antibodies to canine–human chimeras of GPIbα.

(A) Canine–human chimeras of GPIbα, where residue numbers correspond to amino acid sequences of human12 and canine13GPIbα. Canine sequence is represented in black. Chimeras C200 and C268 have been reported previously.11 (B) Binding of anti-GPIbα monoclonal antibodies to CHO cells expressing wild-type GPIbα or the chimeras indicated, as assessed by flow cytometry. −, no binding; (+) approximately 50% maximal binding; +, maximal binding.