Effect of anti-CD9 mAb in TEM assays.
EC monolayers were established in transwell inserts, and TEM assays were carried out with BCECF-labeled A375 cells in the presence of the indicated purified mAb (10 μg/mL) for 24 hours, as described. (A) TEM in the presence of different mAbs against tetraspanin and integrins (B) TEM in the presence of different anti-CD9 mAbs. (C) TEM and bare migration in the presence of anti-CD9 VJ1/10 mAb or its F(ab′)2 fragment. Bars represent the mean ± SD of the percentage migrated TCs in relation to the total number of TCs added to migrate, from 2 parallel experiments in duplicate of 12 independent experiments performed (n = 24) with anti-CD9 VJ1/10 and VJ1/20 and anti-HLA class I W6/32 and of 3 experiments (n = 6) with the other mAbs. *P < .05; **P < .001 compared with medium control or with mAb W6/32.
