American Society of Hematology

Fig. 4.

$Fig. 4. DMT1 isoform II is expressed in the spleen of PHZ-treated mice. / (A) Schematic representation of the 2 DMT1 mRNAs (IRE-containing and non–IRE-containing form generated by alternative splicing) and corresponding proteins (isoform I and II, respectively). The 2 isoforms show distinct 3′ UTR and encode polypeptides with distinct carboxy termini (hatched boxes). The DMT1-NT antibody recognizes the N-terminus of both isoforms I and II (solid boxes), whereas the DMT1-CT antibody was raised against the carboxy-terminal extremity of isoform II. (B) Microsomal spleen fractions (120 μg) from control (−) or PHZ-treated (+) mice were analyzed by immunoblotting with the DMT1-CT antiserum. Membrane proteins (5 μg) from CHO cells or CHO transfectant expressing either a cMyc-tagged DMT1 isoform II (CHO-DMT1) or a cMyc-tagged mNramp1 (CHO-Nramp1) were included as controls. The positions and sizes (in kilodaltons) of molecular mass markers are indicted on the right.$

DMT1 isoform II is expressed in the spleen of PHZ-treated mice.

(A) Schematic representation of the 2 DMT1 mRNAs (IRE-containing and non–IRE-containing form generated by alternative splicing) and corresponding proteins (isoform I and II, respectively). The 2 isoforms show distinct 3′ UTR and encode polypeptides with distinct carboxy termini (hatched boxes). The DMT1-NT antibody recognizes the N-terminus of both isoforms I and II (solid boxes), whereas the DMT1-CT antibody was raised against the carboxy-terminal extremity of isoform II. (B) Microsomal spleen fractions (120 μg) from control (−) or PHZ-treated (+) mice were analyzed by immunoblotting with the DMT1-CT antiserum. Membrane proteins (5 μg) from CHO cells or CHO transfectant expressing either a cMyc-tagged DMT1 isoform II (CHO-DMT1) or a cMyc-tagged mNramp1 (CHO-Nramp1) were included as controls. The positions and sizes (in kilodaltons) of molecular mass markers are indicted on the right.

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