Donor CTLs generate allospecific cytotoxicity following infusion into recipients.

Representative example of ⁵¹Cr lysis results (A) immediately prior to infusion and (B) after transplantation into a DLA-haploidentical recipient. Donor CTLs lysed recipient Con-A blasts at all effector-target ratios studied (solid square with solid line), but had limited or minimal lysis of third-party (solid triangle, broken line) and autologous blasts (X, broken line). GFP⁺ cells were isolated by FACS cell sorting of peripheral blood on day +6 after transplantation and expanded ex vivo as described in “Material and methods.”