Immunogold labeling for CD146 of ECs grown to confluence.

(A) CD146 immunolabeling appears along the intercellular junctions (arrowheads) but is excluded from the electron-dense area corresponding to AJs. A few gold particles are scattered over the cytoplasm, showing the intracellular pool of CD146 (N indicates nucleus; magnification × 63 000). (B) Higher magnification of an AJ. The immunogold labeling for CD146 is absent from the AJ (magnification × 90 000).