CD146 localization at the cell-cell contacts on cultured ECs examined by confocal microscopy.

(A) HUVECs were grown to confluence on coverslips. The cells were stained with anti-CD146 (i), anti–MHC class I used as a control (ii), anti–VE-cadherin (iii), or anti–PECAM-1 (iv). Magnification × 100. (B) Optical sections of endothelial monolayer were obtained with confocal microscopy from the apical (1-3), intermediate (4-6), and basal (7-9) sides of the cells. (i) Labeling for CD146. (ii) VE-cadherin. Magnification × 100.