Fig. 5.
Fig. 5. Maturation capacities of MDCs and PDCs isolated from patients after in vitro culture. / (A,C) The 2 DC subsets CD11c+ILT3+ (MDCs) and CD11c−ILT3+ (PDCs) were sorted from the blood of healthy individuals or (B,D) from leukemic patients and analyzed by flow cytometry after 72 hours of culture with either GM-CSF, IL-4, and CD40L (MDCs) or IL-3 and CD40L (PDCs) for their expression of DC marker CD83 and costimulatory molecules. (A) MDCs and (C) PDCs isolated from healthy volunteers acquired the expression of CD83 and expressed the costimulatory molecules (CD80 and CD86). (B) In leukemic patients, the MDC subset could acquire CD83 and the costimulatory molecules CD80 and CD86. (D) PDCs from leukemic patients never acquired CD83 or costimulatory molecules. Open histograms represent cells stained with isotype-matched control mAbs. Results indicated are representative of those obtained from 4 healthy donors and 5 patients.

Maturation capacities of MDCs and PDCs isolated from patients after in vitro culture.

(A,C) The 2 DC subsets CD11c+ILT3+ (MDCs) and CD11c−ILT3+ (PDCs) were sorted from the blood of healthy individuals or (B,D) from leukemic patients and analyzed by flow cytometry after 72 hours of culture with either GM-CSF, IL-4, and CD40L (MDCs) or IL-3 and CD40L (PDCs) for their expression of DC marker CD83 and costimulatory molecules. (A) MDCs and (C) PDCs isolated from healthy volunteers acquired the expression of CD83 and expressed the costimulatory molecules (CD80 and CD86). (B) In leukemic patients, the MDC subset could acquire CD83 and the costimulatory molecules CD80 and CD86. (D) PDCs from leukemic patients never acquired CD83 or costimulatory molecules. Open histograms represent cells stained with isotype-matched control mAbs. Results indicated are representative of those obtained from 4 healthy donors and 5 patients.

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