Morphologic and confocal microscopy analysis of MDCs and PDCs isolated from AML patients.

CD11c⁺ MDCs were isolated from patient UPN71 and cultured in vitro for 3 days with GM-CSF, IL-4, and CD40L. (A) They display dendritic morphology as shown by interferential contrast transmission microscopy (×100). Three-color immunofluorescence staining was performed. (B) HLA-DR expression is shown in red, (C) CD83 in blue, and (D) DC-LAMP in green. (E) Giemsa staining of freshly isolated PDCs from patient UPN109. Original magnification × 1000.