Immunophenotype of freshly isolated MDCs, PDCs, and leukemic blasts and expression of pre-Tα by leukemic PDCs.

(A) Leukemic MDCs and PDCs were sorted as lin⁻/CD11c⁺/ILT3⁺ (MDCs) and lin⁻/CD11c⁻/ILT3⁺ (PDCs) and compared with blasts obtained from the same patient for the expression of a number of lymphoid, myeloid, costimulatory, and cytokine receptor markers. Open histograms represent cells stained with isotype-matched control mAbs. Data shown were obtained from patient UPN243 and are representative of more than 5 patients with or without DC expansion. (B) Freshly isolated normal and leukemic MDCs and PDCs were analyzed for expression of mRNA for pre-Tα. Data shown are representative of 3 patients.