Fig. 1.
Fig. 1. Detection of circulating DC subsets in the peripheral blood. / PBMCs isolated from healthy volunteers or AML patients were analyzed by flow cytometry after 3-color staining with a combination of FITC-labeled mAbs against lineage markers (CD14, CD16, CD19, and CD56), PE-labeled anti-CD11c, and PC5-labeled ILT3. Two distinct populations of lin−/ILT3+ cells were observed with respect to the expression of CD11c, with the phenotypes of lin−/CD11c+/ILT3+ (MDCs) and lin−/CD11c−/ILT3+ (PDCs). Examples of expansion of the MDC subset for patient UPN109 or the PDC subset for patient UPN223 are shown.

Detection of circulating DC subsets in the peripheral blood.

PBMCs isolated from healthy volunteers or AML patients were analyzed by flow cytometry after 3-color staining with a combination of FITC-labeled mAbs against lineage markers (CD14, CD16, CD19, and CD56), PE-labeled anti-CD11c, and PC5-labeled ILT3. Two distinct populations of lin−/ILT3+ cells were observed with respect to the expression of CD11c, with the phenotypes of lin−/CD11c+/ILT3+ (MDCs) and lin−/CD11c−/ILT3+ (PDCs). Examples of expansion of the MDC subset for patient UPN109 or the PDC subset for patient UPN223 are shown.

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