Fig. 2.
Shear-induced platelet aggregation in PRP.
PRP prepared from heparinized whole blood was subjected to a shear rate of 10 000 s−1 in a cone-and-plate viscometer for 30 seconds at room temperature. Presheared and postsheared samples were fixed in 1% formaldehyde–PBS. Platelet samples were labeled with anti–CD42a-FITC (anti-GPIX), and the extent of platelet aggregation was determined by flow cytometry. *Significant difference from control;P < .05.
