Production of IL-12 (p40) by DC subsets.

Total CD11c⁺ DCs separated by directly conjugated magnetic beads were stimulated for 24 hours with stimulatory anti-CD40 antibodies (CD40 mAb), LPS (10 μg/mL), or SAC. For the detection of intracellular IL-12, DCs were stained with PE-conjugated antibodies recognizing IL-12 (p40/p70) and directly FITC-labeled antibodies to CD11c, CD11b, and B220. Percentage positive cells are indicated in each quadrant. At least 4 similar experiments were performed.