Elevated levels of IFN-α in Flt3L-treated mice.

Mobilization of mice with Flt3L results in elevated levels of IFN-α₂ production from (A) BM and (B) spleen cell populations. Total leukocyte populations from BM and spleen from untreated mice or mice mobilized for 7 days with Flt3L were cultured for 24 hours in medium alone (white columns) or with HSV (black columns, 5 PFU/cell) or SAC (gray columns, 10 μg/mL). Supernatants were harvested and analyzed for their IFN-α₂ content by specific ELISA. Data from 1 of at least 3 experiments yielding similar results are shown. (C) BM cells and (D) splenocytes were prepared from Flt3L+GM-CSF–treated mice, with either CD11c⁺ (white columns) or CD11b⁺ (black columns) cells isolated using directly conjugated magnetic beads (MACS). Cells were cultured for 24 hours in medium alone or with HSV or SAC. Supernatants were collected and analyzed for IFN-α₂ by ELISA. Data from 1 of at least 3 experiments yielding comparable results is shown.