![Fig. 4. BCL11A RNA expression in fetal, adult, and malignant tissues. / (A) Low-level expression of BCL11A RNA in a range of normal adult lymphoid tissues and fetal liver (Clontech Immune System MTN blot; 2 μg poly[A+] mRNA per lane; exposure time, 10 days). A low-level BCL11A transcript of 5.8 kb was seen, with highest levels present in normal lymph node. (B) High-level BCL11Aexpression was seen only in fetal brain (CNS d117) and in patient AS with t(2;14)(p13;q32.3). Predominant BCL11A transcripts of 5.8 kb, 3.5 kb, and 1.5 kb were seen in cases with t(2;14)(p13;q32.3) and in fetal brain. Otherwise, note low-level expression ofBCL11A in malignant B-cell lines (NAB-2 and Granta 452) and in human embryonic kidney fibroblast cell line, HEK-293. Loading control denoted by reprobing of same filters with glyceraldehyde phosphate dehydrogenase (GAPDH) probe shown below. (C) BCL11A mRNA accumulates in germinal center (GC) but not follicular mantle (FM) B cells. Antisense (i) and sense (ii)35S-labeled riboprobes were hybridized to frozen sections of normal human tonsils, or a serial section (iii) was weakly stained with Giemsa to visualize the boundary of the high cellularity FM area. GC cells but not mantle cells exhibited BCL11Aexpression.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/12/10.1182_blood.v98.12.3413/6/h82311835004.jpeg?Expires=1722767617&Signature=MgIDjmMni1d3BFxYK9RSrc~-TUrgwn-fmnDo19jXzQY5xWU6SENmYo4vPIfM2LvBRj724HtB-yxBlJTzG60O7cbGpE0UrQCV6zDmBsI2HXFGu7-qc7uTlsWSUvbEvO8UvKpFqMVvN8vh99UsYNtjdWI7jhN4I~rTMMRs~1MCyno4qcv2kBZKbwbS-BDh0Lg6hOJ6lG3QZ1ixSkrcWRRp7dj0MZRZ4MF95Co1ukft8o7YudBzgheh6REARCOHaahsYWPm4~cyV1h4piDJhTBaxJsSHMnP6WCMfIu2LbdTVk8~eCeEEq4XlZ50gcW4FIz36DG4DuSeCUxF3bNqLH7c3w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
BCL11A RNA expression in fetal, adult, and malignant tissues.
(A) Low-level expression of BCL11A RNA in a range of normal adult lymphoid tissues and fetal liver (Clontech Immune System MTN blot; 2 μg poly[A+] mRNA per lane; exposure time, 10 days). A low-level BCL11A transcript of 5.8 kb was seen, with highest levels present in normal lymph node. (B) High-level BCL11Aexpression was seen only in fetal brain (CNS d117) and in patient AS with t(2;14)(p13;q32.3). Predominant BCL11A transcripts of 5.8 kb, 3.5 kb, and 1.5 kb were seen in cases with t(2;14)(p13;q32.3) and in fetal brain. Otherwise, note low-level expression ofBCL11A in malignant B-cell lines (NAB-2 and Granta 452) and in human embryonic kidney fibroblast cell line, HEK-293. Loading control denoted by reprobing of same filters with glyceraldehyde phosphate dehydrogenase (GAPDH) probe shown below. (C) BCL11A mRNA accumulates in germinal center (GC) but not follicular mantle (FM) B cells. Antisense (i) and sense (ii)35S-labeled riboprobes were hybridized to frozen sections of normal human tonsils, or a serial section (iii) was weakly stained with Giemsa to visualize the boundary of the high cellularity FM area. GC cells but not mantle cells exhibited BCL11Aexpression.
