Fig. 1.
Anti–integrin α2 antibody inhibits platelet spreading on collagen-coated surfaces.
Washed platelets were seeded on collagen-coated surfaces with 10 μg/mL control mouse IgG or with 10 μg/mL anti–integrin α2 antibody (7E10B) for 10 minutes, 30 minutes, and 60 minutes at 30°C. After unbound platelets were removed, the platelets were fixed, then incubated with TRITC-conjugated phalloidin for actin fiber staining.
