The stimulation of allogeneic T cells by BM-donor–derived DCs in the spleens of irradiated recipients.

Ly 5.2⁺ C57BL/6 BM-derived total DCs or CD8α⁺DCs purified from the spleen of recipient mice were cultured in the presence of IL-2 with purified CD4⁺ or CD8⁺ T cells from Balb/c mice. Uptake of ³H-TdR was measured at day 3 of culture. Freshly isolated total splenic DCs or CD8α⁺ DCs from normal mice were used as controls. Background incorporation in the absence of DCs was always fewer than 1000 cpm. Error bars represent the range of triplicate samples.