Fig. 1.
Fig. 1. Schematic structure, expression, and purification of His-M195FANCF. / (A) The structure is drawn approximately to scale. The lengths of the component peptides and key restriction sites are shown. (B) Expression and purification of His-M195FANCF. Lysates from uninduced (−) or IPTG-induced (+) E coli or nickel-agarose–purified protein (Pur; 3 μg) were analyzed by 10% SDS-PAGE and Coomassie blue staining. Arrow indicates the position of the 68.5-kd His-M195FANCF.

Schematic structure, expression, and purification of His-M195FANCF.

(A) The structure is drawn approximately to scale. The lengths of the component peptides and key restriction sites are shown. (B) Expression and purification of His-M195FANCF. Lysates from uninduced (−) or IPTG-induced (+) E coli or nickel-agarose–purified protein (Pur; 3 μg) were analyzed by 10% SDS-PAGE and Coomassie blue staining. Arrow indicates the position of the 68.5-kd His-M195FANCF.

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