Erg GeneBlocs reduce endothelial cell tube formation on Matrigel.
The phenotypic effect of the inhibition of Erg on vascular remodeling was tested in vitro using the Matrigel tube formation assay. HUVECs were treated with Erg GeneBloc (GB566) or the Control GeneBloc (GB478) for 24 hours as described. Then they were trypsinized and counted, and 4 × 104 cells/well were plated onto 15-mm wells containing Matrigel. After 16 hours, tubule formation was analyzed using a Leica 550 image analyser. (A) Low-magnification image (original magnification × 200) of tubes formed by HUVECs treated with control GeneBloc (left) or with Erg GeneBloc (right), showing a marked reduction in tube formation in the Erg GeneBloc-treated cells. (B) The number of branches in each sample was quantified using image analysis (results of one representative experiment are shown here).
