Treatment of HUVECs with the Erg mismatch control GeneBloc 478 does not reduce Erg mRNA levels.

RNA was prepared from HUVECs treated with Erg specific (GB566), Erg mismatch (GB478), and random control (GBC3.3) GeneBlocs for 24, 48, and 72 hours. Shown are levels of Erg mRNA, normalized to actin mRNA, as measured by TaqMan analysis. A ratio of 1 represents Erg mRNA level in untreated HUVECs. Erg expression is inhibited by the Erg-specific GeneBloc but not by the 2 control GB. At the 72-hour point, a small amount of toxicity is observed with the control GeneBloc treatment and may be caused by prolonged treatment with GeneBloc or lipid.