Growth effects of various inhibitors.

Response of representative clones expressing BCR-FGFR1 or ZNF198-FGFR1 to (A) 10 μM manumycin A or 10 μM L-744832, (B) 10 μM or 50 μM PD98059, (C) 40 μM SB202190 or SB203580, (D) 20 μM LY94002. (E) Treatment with SU5402, PD98059 (10 μM), and manumycin A, but not LY294002, results in reduced levels of ERK phosphorylation relative to untreated cells. Total protein was controlled for by probing with anti-MEK; results for L744832 are not shown. (F) Treatment with SB203580 or SB202190 results in reduced levels of phosphorylated CREB relative to untreated cells and total CREB. (G) LY294002 treatment results in reduced AKT phosphorylation relative to untreated cells and total AKT.