![Fig. 7. PLZF expression blocks morphologic changes induced by 1,25(OH)2D3. / Morphometric analysis was done by comparing the nuclear (Nuc) with total-cell area as a function of 1,25(OH)2D3 treatment. (A) Giemsa staining of PLZF45 cells. U937-PLZF45 cells were cultured in the presence or absence of tetracycline (− PLZF and + PLZF) with the indicated treatments. Ligand (1,25(OH)2D3[10−8 M]) was added 16 hours after tetracycline was removed to induce PLZF expression. Total-cell area and nuclear area were calculated on the basis of horizontal and vertical measurements of the diameter of the cell nucleus. Ratios were determined for 10 cells/field. Fields shown were representative of those counted. Original magnifications × 200. (B) Quantitation of the ratios of nuclear-to-cell area in the cells depicted in panel A. The nuclear-to-cell volumes were calculated for 10 separate cells/field shown in panel A, and the results are plotted in panel B (± SD). The x-axis numbers in panel B correspond to the label numbers in panel A.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/12/10.1182_blood.v98.12.3290/6/h82311804007.jpeg?Expires=1726831325&Signature=Ozkv4EajrbJi9xyRzLwtJky6d0QmHz~8PON-pUe-yQR0gjZLESblRUcBjFrJpVwo9HgLTPWXlv9gw1aq8dPXRfSElzT2NNhHww~ZLpGXqvE5QDQH80w4UgPY1WE1brv0EK1n3bmKsjGfAEUdN964mjF~VJ6QWPhbNr7-i2Iw4fTzAQcbL~qYb0yEWo2m5g5c43PiDJzsJCNU3SMmxi7a1xN~QYPKHNJ-~y70ZzeP5PVW78vCfXqes4-Kq3Xj2aCIZZZwQysH0doycyYp920Gj7iFlJnAPOu~YatX698UbAmPB-e2GDy~CW7mtbg66OyddJoFxBo5VWLlUs5nriLebA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PLZF expression blocks morphologic changes induced by 1,25(OH)2D3.
Morphometric analysis was done by comparing the nuclear (Nuc) with total-cell area as a function of 1,25(OH)2D3 treatment. (A) Giemsa staining of PLZF45 cells. U937-PLZF45 cells were cultured in the presence or absence of tetracycline (− PLZF and + PLZF) with the indicated treatments. Ligand (1,25(OH)2D3[10−8 M]) was added 16 hours after tetracycline was removed to induce PLZF expression. Total-cell area and nuclear area were calculated on the basis of horizontal and vertical measurements of the diameter of the cell nucleus. Ratios were determined for 10 cells/field. Fields shown were representative of those counted. Original magnifications × 200. (B) Quantitation of the ratios of nuclear-to-cell area in the cells depicted in panel A. The nuclear-to-cell volumes were calculated for 10 separate cells/field shown in panel A, and the results are plotted in panel B (± SD). The x-axis numbers in panel B correspond to the label numbers in panel A.
