PLZF expression blocks 1,25(OH)₂D₃-inducible cell-surface expression of CD14.

The U937-PLZF45 cell line was cultured in the presence or absence of tetracycline (− PLZF and + PLZF). After 16 hours, 1,25(OH)₂D₃ or ATRA was added, and samples were collected 48 hours later. The samples were fixed and stained with FITC anti-CD14 (A), CD11c (B), or nonspecific FITC antibodies. These samples were then analyzed for surface-marker expression by flow cytometry. FITC nonspecific antibodies did not stain cells (−) or (+) PLZF and were used to set detection levels and size variables for the flow cytometry analyses. Cell viability for all samples was greater than 95% (data not shown). The error shown for induction of cell-surface markers is the result of combining data from 3 separate experiments done in triplicate.