A rescued immunoglobulin from an HCV-associated lymphoma precipitates multiple intracellular forms of the HCV-E2 glycoprotein.

Cell lysates containing each of the HCV glycoproteins were immunoprecipitated with immunoglobulins derived from an anti-E2 mAb (CBH-4G), from the 2 HCV-associated NHL cases, and from a non-HCV NHL control immunoglobulin. Proteins were separated by SDS-PAGE, and blots were probed with an antibody against HCV-E2. Sizes (in kd) of protein markers are indicated on the right, and arrowheads on the left point to the precipitated forms of E2. IP indicates the source of immunoprecipitating immunoglobulin.