Reversal by PU.1 of the effect of aNotch1 on the granulocytic differentiation in 32D cells.

The 32D/aNotch1 cells were infected with a mock virus or a PU.1-containing pMX/IRES-EGFPpuro virus. The cells are shown 4 days after the addition of G-CSF and after Wright-Giemsa staining. Original magnification × 400. Three independent PU.1-expressing clones and control clones derived from each of 2 independent aNotch1-expressing clones were investigated.