Inhibition of erythroid differentiation by aNotch1 in F5-5 cells.

(A) F5-5 cells were infected with mock virus or aNotch1-containing pMX/IRES-EGFPpuro virus. The morphology of clone 33 and a control clone 6 days after the induction for differentiation is demonstrated with 1.5% DMSO or Activin A. Cells were stained with benzidine. Original magnification × 200. (B) Erythroid differentiation was evaluated by the ratio of benzidine-positive cells. Cells were differentiated by DMSO (i) or Activin A (ii). After 6 days, the ratio of benzidine-positive cells was calculated. The results show the mean values of 3 independent experiments and the error bars show SD.