Fig. 1.
The human GM-CSF antagonist E21R behaves as an agonist on mouse cell lines.
Factor-deprived human TF1.8 (A) and mouse hGMR BaF-B03 cells (B) were stimulated with hGM-CSF (●) or E21R (○). Functional antagonism of 0.03 ng/mL GM-CSF with increasing concentrations of E21R on human TF1.8 cells is also shown (▴). Cells were cultured with cytokine for 48 hours and the resulting proliferation was measured by the incorporation of 3H-thymidine. The results are expressed in disintegrations per minute (dpm) and each point represents the mean of triplicate determination with error bars representing 1 SD.
