Protein expression of p53 and Bax during chemotherapy.

Mononuclear cells from patients undergoing chemotherapy were isolated at different time points of treatment, as indicated. Shep cells not stimulated or stimulated with doxorubicin 1 μg/mL for 24 hours served as a positive control. Bax and p53 protein were detected by immunoblot. Actin served as control for the equal loading of protein.