Fig. 7.
Influence of liposomes on degranulation of granulocytes.
Granulocytes were incubated with DMSO (A), with cytochalasin B (0.5 μg/mL)/NfMLP (10−6 M) diluted in DMSO, positive control (B), and with plain liposomes (EPC:EPG:Chol, 1 mM lipid) (C) at 37°C for 30 minutes. Degranulation of MPO from azurophilic granules of neutrophils was measured by registration of the shift of the granulocyte cloud from right to left on the x-axis (decrease of MPO in single neutrophils). Plain EPC:SPS:Chol liposomes and GOLs gave the same results, and incubation with DMSO for 30 minutes had no influence on the signals compared to freshly prepared granulocytes (data not shown).
