Killing of

S aureus by normal and CGD granulocytes. Cells (5 × 10⁵) were incubated at 37°C with S aureus (5 × 10⁵) with and without liposome treatment. GOLs (EPC:SPS:Chol, panel A, and EPC:EPG:Chol, panel B, both 4:3:3 mole ratio, lipid concentration 1 mM) were mixed with granulocytes. Incubation was carried out in the continuous presence of GOLs or after a 15-minute preincubation and subsequent removal of nonphagocytosed GOLs before bacteria were added. Values were set as 100% at the start (0 hours). Shown are the mean values of 3 independent experiments in duplicates (CV [coefficient of variation] < 0.27); ▪ indicates normal granulocytes; ●, CGD granulocytes (both without liposome treatment); ▴, liposomes; ▾, CGD granulocytes with continuous incubation of liposomes; ♦, CGD granulocytes preincubated with liposomes. Results of 2-groupt test are marked with asterisk; P < .05.