MLL/AF4 is expressed by μ⁻ and μ⁺ BLIN-3 cells.

(A) RT-PCR analysis of MLL/AF4 expression. cDNA generated from μ⁻ BLIN-3 and μ⁺ BLIN-3 sublines was amplified using primers that specifically amplify MLL/AF4 transcripts.32 PCR products were Southern blotted with an oligonucleotide probe internal to the amplified sequence. GAPDH was used as a control for cDNA integrity. H₂O, no cDNA template; RT, no reverse transcriptase. (B) Western blot analysis of AF4 expression in BLIN-3 cells. Whole-cell lysates were prepared, and 80 μg protein per lane was electrophoresed on a 6% SDS-PAGE gel and subsequently transferred to nitrocellulose. Membranes were probed with mouse anti–human AF4 mAb. The 2 blots represent separate gels.