Fig. 10.
Cross-linking the BLIN-3 pre-BCR leads to an increase in cell number.
μ+ BLIN-3 cells (3.0 × 104) were cultured on BM stromal cells supplemented with 10 ng/mL IL-7 in the presence or absence of the indicated antibodies at 10 μg/mL. BLIN-3 cell numbers were quantified on days 7, 14, and 17 using a microsphere flow cytometric assay.23 Inclusion of anti-μ resulted in a 14-fold increase in cell number by day 17 (compared to day 0). In contrast, medium alone, control IgG1, and anti–major histocompatibility class I (anti-–MHC class I) resulted in 5.5-, 4.6-, and 6.7-fold increases, respectively. Each symbol represents the mean ± SD of triplicate values.
