Prostratin induces differentiation of the monocytic cell-line, THP-1.

THP-1, a suspension myeloid cell line, was treated with 0.3 μM PMA (A,B) or 0.3 μM prostratin (C,D). PMA-treated cells assumed the morphology typical of maturing macrophage 5 days after treatment as shown in panel B; prostratin-treated cells appeared to revert to a nonadherent phenotype as indicated in panel D. These observations are representative of 3 independent experiments.