Fig. 1.
Prostratin induces differentiation of peripheral blood and marrow mononuclear phagocytes.
Equivalent numbers (2 × 106 cells/mL) of human PBMCs or murine bone marrow cultures were plated and treated with PHA/IL-2 (A,C) or 0.263 μM prostratin (B,D). Numerous adherent mononuclear phagocytes of darkened and irregular flattened shape were apparent in both of the prostratin-treated cultures within hours following exposure to the compound (magnified inset, bottom right corner with select monocytes denoted by arrows). Cells were photographed 24 hours after treatment. These observations are representative of multiple experiments and microscopic fields.
