Fig. 1.
High-resolution DNA fingerprint analysis of cell lines Jijoye and HS-Sultan.
A multiple-locus DNA profile of the indicated cell lines was generated using 10 μg of DNA digested to completion (HinfI), size-separated on agarose gels and blotted on positively-charged nylon membranes. After hybridization with a digoxigenin-labelled (GTG)5 oligonucleotide as probe, a chemoluminescent detection of (GTG)5 was carried out and the blot exposed for 15 minutes to X-ray films. Molecular weight markers were used for size determination. Cervix carcinoma cell line HELA was used as positive control. The methods used have been described in details elsewhere.11,13
