Cell surface TFPI activity.

Lp(a) decreases endothelial cell surface TFPI activity in a dose-dependent manner in vitro in the presence (shade bars) or absence (filled bars) of L-PLG (2 μM). TFPI activity on human endothelial cells was measured following incubation for 1 hour at 37°C in the presence of varying concentrations of Lp(a). Following incubation the cells were washed with TBS buffer and cell surface TFPI activity assessed. All data presented are the means of 3 separate experiments and the asterisk indicates P < .01.