Fig. 5.
The p53-independent expression of p21 and p27 in megakaryocytes.
Megakaryocytes were obtained from p53−/−lin− murine cells cultured for 4 days with SCF and PEG-rHu MGDF. Double immunostaining was performed with mouse anti-p21 and rabbit anti-p27 antibodies followed by incubation with donkey TRITC antimouse and FITC antirabbit F(ab′)2 fragments, respectively. Nuclei were counterstained with DAPI. Bar, 10 μm. More than 500 megakaryocytes were examined in 3 experiments.
