American Society of Hematology

Fig. 3.

$Fig. 3. Rb expression during polyploidization. / Human megakaryocytes were sorted as in Figure 1. A similar technique of sorting was used for murine megakaryocytes at day 2 of culture, but only the cell fraction with at least 8N was recovered. Western blot was performed with an anti-Rb mAb, which recognizes both the human (panel A) and murine (panel B) molecules. The human epithelial cell line SAOS (panel A) and the murine stromal cell line MS-5 (panel B) were used as controls. Western blots are from a representative experiment (n = 3).$

Rb expression during polyploidization.

Human megakaryocytes were sorted as in Figure 1. A similar technique of sorting was used for murine megakaryocytes at day 2 of culture, but only the cell fraction with at least 8N was recovered. Western blot was performed with an anti-Rb mAb, which recognizes both the human (panel A) and murine (panel B) molecules. The human epithelial cell line SAOS (panel A) and the murine stromal cell line MS-5 (panel B) were used as controls. Western blots are from a representative experiment (n = 3).

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