Fig. 3.
Fig. 3. Tyrosine phosphorylation of JAK2 in primary fetal liver cells retrovirally expressing BCR-ABL. / (A) Flow cytometry analysis of GFP expression of wild-type fetal liver cells infected with either MSCV-P210-IRES-GFP or the control vector MSCV-IRES-GFP. Live cells were gated. (B) Equal number of serum-starved cells from A were stimulated with or without Epo (100 u/mL). JAK2 immunoprecipitates were run on an 8% SDS-PAGE and probed with antiphosphotyrosine antibody (4G10) (upper panel, i: 2 hours exposure; ii: 10 minutes exposure) or anti-JAK2 antisera (lower panel). The arrow indicates JAK2; IP, immunoprecipitation; WB, Western blot.

Tyrosine phosphorylation of JAK2 in primary fetal liver cells retrovirally expressing BCR-ABL.

(A) Flow cytometry analysis of GFP expression of wild-type fetal liver cells infected with either MSCV-P210-IRES-GFP or the control vector MSCV-IRES-GFP. Live cells were gated. (B) Equal number of serum-starved cells from A were stimulated with or without Epo (100 u/mL). JAK2 immunoprecipitates were run on an 8% SDS-PAGE and probed with antiphosphotyrosine antibody (4G10) (upper panel, i: 2 hours exposure; ii: 10 minutes exposure) or anti-JAK2 antisera (lower panel). The arrow indicates JAK2; IP, immunoprecipitation; WB, Western blot.

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