URO-D protein and mRNA levels present in EBV-transformed lymphocytes.

Total URO-D mRNA and URO-D protein were quantified as described in “Patients and methods.” Densitometry values were normalized to the levels of β-actin for mRNA and B56 phosphatase 2A subunit for total protein. The average from 3 separate experiments is shown. Error bars represent the standard deviation. The control value represents the average of 6 nonporphyric EBV-transformed cell lines. Solid bars represent URO-D mRNA levels, and hatched bars represent URO-D protein level. In all mutants except one, the relative amount of URO-D mRNA exceeded the protein level. The insertion mutant (Glu218insT) generated approximately equivalent amounts of mRNA and protein.