Effect of PTPεC on the onset of IL-6–induced STAT3 tyrosine phosphorylation.

M1 stable clones were treated with IL-6 at 10 ng/mL for the times indicated and lysed. Phosphorylated STAT3 was detected by Western blotting with an antibody specific to tyrosine-phosphorylated STAT3 (upper panels). Blots were stripped and reprobed with the anti-STAT3 antibody (lower panels).