Fig. 1.
Expression of SOCS mRNAs in M1-PTPε clones.
Total RNA was prepared from M1-stable clones cultured for 4 hours in the absence or presence of 25 ng/mL IL-6 and analyzed for expression of SOCS-1, SOCS-3, and β-actin mRNA by Northern blotting. RNA from NIH3T3 cells treated with 25 ng/mL IFN-γ was also analyzed for SOCS-3 expression as a positive control.
