American Society of Hematology

Fig. 9.

$Fig. 9. Caspase inhibitor, z-VAD-fmk, does not suppress G2/M arrest but inhibits PS externalization. / BSO-pretreated cells were analyzed 12 and 24 hours after incubation with medium alone (control) or DBBF-Hb (50 μM) and GOX (10 mU/mL) with and without pretreatment with z-VAD-fmk. (A) DNA content histograms with calculated fractions of G1, S, and G2/M from a representative experiment are shown. The fraction of sub-G1 was calculated as a percentage of total gated events. (B) Typical histograms of annexin V staining of the PI-negative cell population (R1 + R2). Mean fluorescence of the gated cell population is shown for each panel.$

Caspase inhibitor, z-VAD-fmk, does not suppress G2/M arrest but inhibits PS externalization.

BSO-pretreated cells were analyzed 12 and 24 hours after incubation with medium alone (control) or DBBF-Hb (50 μM) and GOX (10 mU/mL) with and without pretreatment with z-VAD-fmk. (A) DNA content histograms with calculated fractions of G1, S, and G2/M from a representative experiment are shown. The fraction of sub-G1 was calculated as a percentage of total gated events. (B) Typical histograms of annexin V staining of the PI-negative cell population (R1 + R2). Mean fluorescence of the gated cell population is shown for each panel.

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