Redox cycling of DBBF-Hb by glucose oxidase leads to morphologic changes in endothelial cells.

Endothelial cells were incubated with FBS-free medium alone (A, B), 10 mU/mL GOX (C, D) or 50 μM DBBF-Hb and 10 mU/mL GOX (E, F). Cells were viewed by phase-contrast microscopy and photographed (×20 objective). Photomicrographs were taken after 8 (left panels) and 20 (right panels) hours. (E) Detached cells out of the plane of focus appear bright. Arrow indicates a cell in the process of rounding and detaching. (F) Arrow points to cellular debris.