Effect of deoxygenation of AA and SS cells on membrane PTP activity and thiol (SH) content.

AA or SS cells were incubated for 30 minutes under either oxygenated (O2) or deoxygenated conditions (N2). Membranes were prepared by hypotonic lysis of the cells. PTP activity and thiol content were measured from the release of p-nitrophenol from pNpp and from the reduction of DTNB, respectively. Data are means ± SE of 4 to 6 independent experiments carried out in duplicate. *Significantly different from oxygenated control.