Fig. 1.
![Fig. 1. Effect of deoxygenation of SS cells on Lyn and Syk activity. / SS cells were incubated either under oxygenated (−) or deoxygenated conditions (+). After immunoprecipitation with anti-Lyn (A,B) or anti-Syk (C,D) antibody and IC assays with [γ-32P] ATP, the reaction products were separated by gel electrophoresis and either visualized by PhosphorImager (A,C) or transferred to Immobilon membranes for immunoblotting with anti-Lyn or anti-Syk antibody (B,D). In C, the lower band (38 kd), whose phosphorylation increased in deoxygenated samples, was generated by proteolysis of phosphorylated Syk (72 kd), as demonstrated by immunoblotting (data not shown).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/10/10.1182_blood.v98.10.3121/5/h82211738001.jpeg?Expires=1724262327&Signature=wH-f8eNEwPJd0LJPvQexCP4PDZPGnsrx4CDoEXo9ArM5K54SGUIfz22Sq7-3FoUAgIcLyDCL2lPWEmysPduXl6KRQVmdqnBBuwHK25GFPhGDaBo8zDeFL3mw0PHm58S2aidP5KxId09KgvWcQvYnIxscdHtyuvGkEpLbHIbWL3-r6Rjt1oZk-ucXbfUleTQhL4uNU1B8KyiGvIV74f0WFOqFmh04xURBS6XNNi-4rvsqVv5B7KTazL9Shl3ikX74rXSuowv018X4DHcHX3p1bqygVDmaXxYJ3U5lZiRriCYe0fQQPW7r5Cx8PyWS419739OC5F1JfN0ASJRuHGe~NQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of deoxygenation of SS cells on Lyn and Syk activity.
SS cells were incubated either under oxygenated (−) or deoxygenated conditions (+). After immunoprecipitation with anti-Lyn (A,B) or anti-Syk (C,D) antibody and IC assays with [γ-32P] ATP, the reaction products were separated by gel electrophoresis and either visualized by PhosphorImager (A,C) or transferred to Immobilon membranes for immunoblotting with anti-Lyn or anti-Syk antibody (B,D). In C, the lower band (38 kd), whose phosphorylation increased in deoxygenated samples, was generated by proteolysis of phosphorylated Syk (72 kd), as demonstrated by immunoblotting (data not shown).
