Induction of TF mRNA expression in lean mice by insulin.

Male lean mice (C57BL/6J^+/+) 6 to 8 weeks old were injected intraperitoneally with 10 U human insulin (Himulin) or saline, and various tissues were removed 3, 6, and 24 hours later. Total RNA was prepared and analyzed for TF gene expression by quantitative RT-PCR. n = 3, mean ± SD.