Fig. 5.
![Fig. 5. Effect of lactacystin on the synthesis of normal VWF and Cys1149Arg. / BHK cells expressing normal VWF (A) or Cys1149Arg (B) treated without (control) or with lactacystin were pulse-labeled with [35S]cysteine and [35S]methionine for 30 minutes and chased in unlabeled complete medium for the times indicated. VWF was immunoprecipitated from cell lysates and conditioned medium for analysis by SDS-PAGE on 5% polyacrylamide gels and autoradiography as described under “Materials and methods.” The positions of pro-VWF (about 350 kd) and mature VWF subunits (about 250 kd) are indicated at the right.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/10/10.1182_blood.v98.10.2973/5/h82211777005.jpeg?Expires=1726836230&Signature=Drz63hQ8RG14NqsqvN~FdwKSqyjWxU4Nx9WaIZM4kWXkgX4m03cgmIilxMqonOSmNuG6Vr4fxbTxWHzncK4uuDgfssHL0tZUPX4dZpQNjdvSJXNolkZU6p-9JRY~aURlhGRUUtywuEpnTTf2L5tgcg2DG3HsuVX4~Iv54NlQKKxnm~~lvPJ2b2PWZ9~UwA-meZ9F~6OsjVspYiWwhifGwzcwgR9FGw0SZNhLrmk3i93HCwUS3KpAbZ4OLQ87QFKElor9kx-o8u2zGH5CYlEhGDFlWBgrchUlYZtAw6KRX8xbtzbNThetVkTaue8oLypEw2dMB5hIdfY694XLe7Cn7w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of lactacystin on the synthesis of normal VWF and Cys1149Arg.
BHK cells expressing normal VWF (A) or Cys1149Arg (B) treated without (control) or with lactacystin were pulse-labeled with [35S]cysteine and [35S]methionine for 30 minutes and chased in unlabeled complete medium for the times indicated. VWF was immunoprecipitated from cell lysates and conditioned medium for analysis by SDS-PAGE on 5% polyacrylamide gels and autoradiography as described under “Materials and methods.” The positions of pro-VWF (about 350 kd) and mature VWF subunits (about 250 kd) are indicated at the right.
